Larry Daley is a divorced father who can't seem to keep a job for more than a week. As a last resort, Larry applies for a job as a night shift security guard at the American Museum of Natural History. However, a seemingly easy job turns out to be an adventure when he finds that an ancient curse causes the "inhabitants" of the museum to come to life at night.

Based upon the book by Milan Trenc. Originally released as a motion picture in 2006. Special features: Commentary by director Shawn Levy; commentary by writers Robert Ben Garant and Thomas Lennon.